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anti hcn2  (Alomone Labs)


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    Structured Review

    Alomone Labs anti hcn2
    Anti Hcn2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+hcn2/Anti-HCN2+Antibody/pm40764992-92-24-26
    Average 94 stars, based on 78 article reviews
    anti hcn2 - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Incubation:

    Article Title: Cardiac electrophysiological remodeling associated with enhanced arrhythmia susceptibility in a canine model of elite exercise
    Article Snippet: .. After the incubation period, cells were labeled overnight at 4°C with anti-KChIP2 (Alomone, #APC-142, RRID: AB_2756744 ), anti-Kv4.3 (Alomone, #APC-017, RRID: AB_2040178 ), anti-HCN1 (Alomone, #APC-056, RRID: AB_2039900 ), anti-HCN2 (Alomone, #APC-030, RRID: AB_2313726 ), and anti-HCN4 (Alomone, #APC-052, RRID: AB_2039906 ) primary antibodies diluted 1:50. .. The next day, cells were incubated with goat anti-rabbit IgG Alexa Fluor 488 (ThermoFisher, A-11034, RRID: AB_2576217 ) secondary antibody (dilution: 1:500, ThermoFisher).

    Article Title: The cGMP-Dependent Protein Kinase II Is an Inhibitory Modulator of the Hyperpolarization-Activated HCN2 Channel
    Article Snippet: Subsequently, cells were quenched with 50 mM ammonium chloride, permeabilized with 0.1% Triton X-100 (Roth) and blocked with 2% fetal bovine serum and 2% bovine serum albumin containing 0.2% cold water fish gelatin. .. Primary neurons were incubated over night at 4°C with anti-HCN2 (Alomone) and anti-myc (clone 9B11, Cell Signaling) diluted in 5% Chemiblocker (Chemicon). ..

    Labeling:

    Article Title: Cardiac electrophysiological remodeling associated with enhanced arrhythmia susceptibility in a canine model of elite exercise
    Article Snippet: .. After the incubation period, cells were labeled overnight at 4°C with anti-KChIP2 (Alomone, #APC-142, RRID: AB_2756744 ), anti-Kv4.3 (Alomone, #APC-017, RRID: AB_2040178 ), anti-HCN1 (Alomone, #APC-056, RRID: AB_2039900 ), anti-HCN2 (Alomone, #APC-030, RRID: AB_2313726 ), and anti-HCN4 (Alomone, #APC-052, RRID: AB_2039906 ) primary antibodies diluted 1:50. .. The next day, cells were incubated with goat anti-rabbit IgG Alexa Fluor 488 (ThermoFisher, A-11034, RRID: AB_2576217 ) secondary antibody (dilution: 1:500, ThermoFisher).

    Western Blot:

    Article Title: Tau‐mediated synaptic dysfunction is coupled with HCN channelopathy
    Article Snippet: .. The following primary antibodies were used in this study: mouse PHF‐1 (generously provided by Prof Peter Davies, western blot, 1/2000, RRID:AB_2315150), rabbit anti‐Tau (Agilent Dako, western blot, 1/5000, RRID:AB_10013724), mouse anti‐NSE (ThermoFisher Scientific, western blot, 1/5000, RRID:AB_560397), mouse anti‐GAPDH (Santa Cruz, western blot, 1/5000, RRID:AB_627679), mouse anti‐synapsin 1 (EMD Millipore, western blot, 1/1000, cat. number: MABN894), mouse anti‐synaptotagmin (BD Biosciences, western blot, 1/1000, RRID:AB_397810), rabbit anti‐postsynaptic density protein‐95 (Cell Signaling, western blot, 1/2000, RRID:AB_1264242), rabbit anti‐HCN1 (Alomone Labs, immunostaining, 1/100, RRID:AB_2039900), guinea pig anti‐HCN1 (Alomone Labs, immunostaining, 1/50, RRID:AB_2756625), rabbit anti‐HCN1 (Novus Biologicals, western blot, 1/1000, NBP2‐14084), mouse anti‐HCN2 (Novus Biologicals, western blot, 1/1000, NBP2‐12895), rabbit anti‐HCN2 (Alomone Labs, immunostaining, 1/100, RRID:AB_2313726), rabbit anti‐HCN3 (Alomone Labs, immunostaining, 1/100, RRID:AB_2039904), rabbit anti‐HCN3 (Alomone Labs, western blot, 1/1000, RRID:AB_2039904), rabbit anti‐HCN3 (Alomone Labs, immunostaining, 1/50, AB_2756742), guinea pig anti‐HCN4 (Alomone Labs, immunostaining, 1/50, RRID:AB_2340957). .. For immunolabeling, fluorescently labeled highly cross‐absorbed secondary antibodies were purchased from either ThermoFisher Scientific or Jackson ImmunoResearch and used at 1/1000 dilution.

    Cell-Signaling:

    Article Title: Tau‐mediated synaptic dysfunction is coupled with HCN channelopathy
    Article Snippet: .. The following primary antibodies were used in this study: mouse PHF‐1 (generously provided by Prof Peter Davies, western blot, 1/2000, RRID:AB_2315150), rabbit anti‐Tau (Agilent Dako, western blot, 1/5000, RRID:AB_10013724), mouse anti‐NSE (ThermoFisher Scientific, western blot, 1/5000, RRID:AB_560397), mouse anti‐GAPDH (Santa Cruz, western blot, 1/5000, RRID:AB_627679), mouse anti‐synapsin 1 (EMD Millipore, western blot, 1/1000, cat. number: MABN894), mouse anti‐synaptotagmin (BD Biosciences, western blot, 1/1000, RRID:AB_397810), rabbit anti‐postsynaptic density protein‐95 (Cell Signaling, western blot, 1/2000, RRID:AB_1264242), rabbit anti‐HCN1 (Alomone Labs, immunostaining, 1/100, RRID:AB_2039900), guinea pig anti‐HCN1 (Alomone Labs, immunostaining, 1/50, RRID:AB_2756625), rabbit anti‐HCN1 (Novus Biologicals, western blot, 1/1000, NBP2‐14084), mouse anti‐HCN2 (Novus Biologicals, western blot, 1/1000, NBP2‐12895), rabbit anti‐HCN2 (Alomone Labs, immunostaining, 1/100, RRID:AB_2313726), rabbit anti‐HCN3 (Alomone Labs, immunostaining, 1/100, RRID:AB_2039904), rabbit anti‐HCN3 (Alomone Labs, western blot, 1/1000, RRID:AB_2039904), rabbit anti‐HCN3 (Alomone Labs, immunostaining, 1/50, AB_2756742), guinea pig anti‐HCN4 (Alomone Labs, immunostaining, 1/50, RRID:AB_2340957). .. For immunolabeling, fluorescently labeled highly cross‐absorbed secondary antibodies were purchased from either ThermoFisher Scientific or Jackson ImmunoResearch and used at 1/1000 dilution.

    Article Title: The cGMP-Dependent Protein Kinase II Is an Inhibitory Modulator of the Hyperpolarization-Activated HCN2 Channel
    Article Snippet: Subsequently, cells were quenched with 50 mM ammonium chloride, permeabilized with 0.1% Triton X-100 (Roth) and blocked with 2% fetal bovine serum and 2% bovine serum albumin containing 0.2% cold water fish gelatin. .. Primary neurons were incubated over night at 4°C with anti-HCN2 (Alomone) and anti-myc (clone 9B11, Cell Signaling) diluted in 5% Chemiblocker (Chemicon). ..

    Immunostaining:

    Article Title: Tau‐mediated synaptic dysfunction is coupled with HCN channelopathy
    Article Snippet: .. The following primary antibodies were used in this study: mouse PHF‐1 (generously provided by Prof Peter Davies, western blot, 1/2000, RRID:AB_2315150), rabbit anti‐Tau (Agilent Dako, western blot, 1/5000, RRID:AB_10013724), mouse anti‐NSE (ThermoFisher Scientific, western blot, 1/5000, RRID:AB_560397), mouse anti‐GAPDH (Santa Cruz, western blot, 1/5000, RRID:AB_627679), mouse anti‐synapsin 1 (EMD Millipore, western blot, 1/1000, cat. number: MABN894), mouse anti‐synaptotagmin (BD Biosciences, western blot, 1/1000, RRID:AB_397810), rabbit anti‐postsynaptic density protein‐95 (Cell Signaling, western blot, 1/2000, RRID:AB_1264242), rabbit anti‐HCN1 (Alomone Labs, immunostaining, 1/100, RRID:AB_2039900), guinea pig anti‐HCN1 (Alomone Labs, immunostaining, 1/50, RRID:AB_2756625), rabbit anti‐HCN1 (Novus Biologicals, western blot, 1/1000, NBP2‐14084), mouse anti‐HCN2 (Novus Biologicals, western blot, 1/1000, NBP2‐12895), rabbit anti‐HCN2 (Alomone Labs, immunostaining, 1/100, RRID:AB_2313726), rabbit anti‐HCN3 (Alomone Labs, immunostaining, 1/100, RRID:AB_2039904), rabbit anti‐HCN3 (Alomone Labs, western blot, 1/1000, RRID:AB_2039904), rabbit anti‐HCN3 (Alomone Labs, immunostaining, 1/50, AB_2756742), guinea pig anti‐HCN4 (Alomone Labs, immunostaining, 1/50, RRID:AB_2340957). .. For immunolabeling, fluorescently labeled highly cross‐absorbed secondary antibodies were purchased from either ThermoFisher Scientific or Jackson ImmunoResearch and used at 1/1000 dilution.

    Comparison:

    Article Title: Spinal Nerve Axotomy: Effects on I h In Vivo and HCNs in DRG Neurons.
    Article Snippet: .. Anti-HCN1 primary antibody (Alomone, Jerusalem, Israel, 1:1000) was used for image analysis of immunoreactivity, and both anti-HCN1 and anti-HCN2 (Alomone company, 1:2000) were used for general image comparison. ..

    Article Title: Spinal Nerve Axotomy: Effects on I h In Vivo and HCNs in DRG Neurons
    Article Snippet: .. Anti-HCN1 primary antibody (Alomone, Jerusalem, Israel, 1:1000) was used for image analysis of immunoreactivity, and both anti-HCN1 and anti-HCN2 (Alomone company, 1:2000) were used for general image comparison. ..



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    HCN, an I h ion channel, localized to both inner and outer surfaces of calyces. (A) Section from the CZ of a female rat crista immunolabeled for calretinin ( Calret , green ) and <t>HCN2</t> (red) in several calretinin‐positive calyx‐only afferents; a single calretinin‐negative dimorphic calyx ( D ) is present. In (A’), the calretinin label seen in (A) has been removed to show light HCN2 labeling of all calyces, including the dimorphic calyx ending. (B, B’) Same immunolabeling as in (A, A’), but from the PZ. A single type II hair cell ( II ) and several dimorphic ( D ) calyces are present; the calyces are slightly more heavily labeled for HCN2 than are those in the CZ; the type II hair cell is very lightly HCN2 labeled. HCN2 label is found on both the inner and outer surfaces of the calyx ( arrows ). Scale bar (for all four panels) = 5 µm.
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    Image Search Results


    HCN, an I h ion channel, localized to both inner and outer surfaces of calyces. (A) Section from the CZ of a female rat crista immunolabeled for calretinin ( Calret , green ) and HCN2 (red) in several calretinin‐positive calyx‐only afferents; a single calretinin‐negative dimorphic calyx ( D ) is present. In (A’), the calretinin label seen in (A) has been removed to show light HCN2 labeling of all calyces, including the dimorphic calyx ending. (B, B’) Same immunolabeling as in (A, A’), but from the PZ. A single type II hair cell ( II ) and several dimorphic ( D ) calyces are present; the calyces are slightly more heavily labeled for HCN2 than are those in the CZ; the type II hair cell is very lightly HCN2 labeled. HCN2 label is found on both the inner and outer surfaces of the calyx ( arrows ). Scale bar (for all four panels) = 5 µm.

    Journal: The Journal of Comparative Neurology

    Article Title: Distribution of Voltage‐Gated Sodium Channels and Scaffolding Proteins on Vestibular Calyx Ending Delineates the Axon Initial Segment

    doi: 10.1002/cne.70127

    Figure Lengend Snippet: HCN, an I h ion channel, localized to both inner and outer surfaces of calyces. (A) Section from the CZ of a female rat crista immunolabeled for calretinin ( Calret , green ) and HCN2 (red) in several calretinin‐positive calyx‐only afferents; a single calretinin‐negative dimorphic calyx ( D ) is present. In (A’), the calretinin label seen in (A) has been removed to show light HCN2 labeling of all calyces, including the dimorphic calyx ending. (B, B’) Same immunolabeling as in (A, A’), but from the PZ. A single type II hair cell ( II ) and several dimorphic ( D ) calyces are present; the calyces are slightly more heavily labeled for HCN2 than are those in the CZ; the type II hair cell is very lightly HCN2 labeled. HCN2 label is found on both the inner and outer surfaces of the calyx ( arrows ). Scale bar (for all four panels) = 5 µm.

    Article Snippet: HCN2 , NeuroMab , 75‐111 RRID:AB_2279449 , , , , , , Datasheet.

    Techniques: Immunolabeling, Labeling